Detailed Description
5-Amino-1MQ is a low-molecular-weight quaternary ammonium compound utilized in laboratory research to examine NNMT-mediated biochemical processes. NNMT catalyzes the methylation of nicotinamide, influencing intracellular nicotinamide availability and intersecting with NAD-related metabolic and methylation pathways.
Because NNMT activity regulates both nicotinamide clearance and methyl donor utilization, selective NNMT inhibitors such as 5-Amino-1MQ are employed as pathway-specific research tools. These compounds enable controlled investigation of enzyme kinetics, metabolic flux, and signaling network integration within experimental systems, without implying physiological or clinical outcomes.
Biochemical Properties
Properties
Synthetic small-molecule NNMT inhibitor
Structure
Aromatic quinolinium core with amino substitution
Class
Small-molecule enzyme inhibitor
Behavior
Interacts with nicotinamide N-methyltransferase in biochemical and cellular assay systems
Solubility or Stability
Highly water-soluble under standard laboratory preparation conditions
Chemically stable in neutral aqueous environments
Identifiers
Chemical Name: 5-Amino-1-methylquinolinium
CAS Number: 685079-15-6
Molecular Formula: C₁₀H₁₁N₂⁺
Molecular Weight: 159.21 g/mol
PubChem CID: 950107
Structural & Molecular Data
Amino Acid Sequence: Not applicable
Molecular Formula: C₁₀H₁₁N₂⁺
Structural Notes
5-Amino-1MQ is a low-molecular-weight ionic compound with a planar aromatic structure. The positively charged quinolinium core contributes to predictable aqueous solubility and consistent interaction behavior in enzymatic and biochemical assay environments.
Scientific Description
Overview
5-Amino-1MQ is investigated as a molecular probe for NNMT-regulated nicotinamide metabolism and associated biochemical signaling pathways.
Biochemical Characteristics
In preclinical research systems, 5-Amino-1MQ functions as a competitive inhibitor of NNMT. Inhibition of NNMT alters nicotinamide methylation dynamics, allowing examination of downstream effects on nicotinamide availability, methyl donor balance, and related metabolic signaling pathways.
Experimental Research Scope
NNMT enzymatic activity and inhibition kinetics
Nicotinamide metabolism and clearance pathways
NAD-associated metabolic signaling networks
Cellular energy metabolism in preclinical models
Methylation-dependent biochemical processes
Molecular and Signaling Context
NNMT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to nicotinamide, producing methylated nicotinamide derivatives. This reaction intersects with NAD salvage pathways and cellular methylation balance, positioning NNMT as a key regulatory node in metabolic research.
Summary of Preclinical Investigation
5-Amino-1MQ has been evaluated in cellular and animal research models to characterize NNMT inhibition, enzyme regulation, and pathway-level metabolic signaling. These investigations focus on mechanistic understanding rather than physiological or clinical endpoints.
Form & Analytical Testing
Form: Lyophilized solid
Appearance: Yellow–orange crystalline solid
Analytical methods: HPLC, MS
Identity and purity verified prior to release